Is IgG Negatively Charged? Understanding Its Charge Properties
Discover if IgG is negatively charged and learn about its isoelectric point and implications in chromatography.
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IgG is generally adhered to by cation-exchange chromatography methods, which implies it is negatively charged due to its isoelectric point typically ranging from 6-9. This reflects its overall net negative charge at neutral pH.
FAQs & Answers
- What is the isoelectric point of IgG? IgG has an isoelectric point that typically ranges from 6 to 9.
- What methods are used to analyze IgG? Cation-exchange chromatography is commonly used to analyze and purify IgG.
- Why is the charge of IgG important? The charge of IgG affects its behavior during purification processes and its interactions with other biomolecules.
- How does pH affect IgG charge? At neutral pH, IgG generally exhibits a net negative charge due to its isoelectric point.