Questions in this topic
- Why do you soak PVDF membrane in methanol?
- Can I leave secondary antibody overnight?
- What is BSA blocking?
- What is gun cotton made of?
- What is in transfer buffer?
- What is nitrocellulose made out of?
- What is nitrocellulose membrane?
- What is PVDF pipe used for?
- What is the function of TAE buffer in gel electrophoresis?
- What is the purpose of blocking solution?
- What is the purpose of Laemmli buffer?
- What is the purpose of the stacking gel?
- Why do proteins migrate from the gel to the membrane?
- Why do we add methanol in transfer buffer?
- Why is BSA a blocking agent?
- Why is glycine used in running buffer?
- Why is PVDF membrane activated by methanol?
- What is a 1% solution?
- Is nitrocellulose toxic?
- Is cordite still used?
- Can transfer buffer be reused?
- Can you incubate secondary antibody overnight?
- Do you need to wash after blocking?
- Do you wash after blocking?
- How do you make a 1% glucose solution?
- How do you make a 10% BSA solution?
- How do you make a 2% BSA solution?
- How do you make a 3 BSA solution?
- How do you make a 5% milk solution?
- How do you make TBS tweens?
- How do you store a Western blot membrane after transfer?
- How do you store PVDF membranes?
- How do you use a PVDF membrane?
- How long can you block a membrane for?
- How long can you leave primary antibody?
- How much methanol is in a transfer buffer?
- Why nitrocellulose membrane is used in blotting?